Sure you can! You will first be prompted to create a new List, since the Listing Builder can only work with existing keywords lists. You will then use those keywords as reference to start building the content for your Amazon listing.
The first step will be to click the Create new List button:
You will be then be taken to the My List section where you can create a new list and start adding keywords to it from Keyword Scout. You'll be prompted to give your list a name, then you can click Create List
Once that's ready you will be taken to an empty list the next step is to add some keywords to it. To do that click on the Keyword Scout to search and select relevant keyword to add to your new list:
Tick the boxes next to the keywords to select the ones you want to add to your list, and then hover over the top box on that column to see the options menu.
Then click on the Add to Keyword List option and you will see all the lists you can add the keywords to:
If you go back to your Listing Builder now and click on the Create new Listing option, you should see your new list available to use: